Nexperion - Cryo-Tomography Workshop

The Electron Microscopy Facility of the Vienna Biocenter Core Facilities (VBCF, Vienna, Austria), the Schur Lab at Institute of Science and Technology Austria, and Nexperion – Solutions for Electron Microscopy (Vienna, Austria) have jointly organised an international

Advanced Workshop on Cryo-Electron Tomography

Saturday, May 7 – Friday, May 13, 2022

Vienna, Austria, Europe (on-site)

for students, postdoctoral staff, scientists, and group leaders from academia and industry. This workshop is supported by the Electron Microscopy Facility at ISTA.

Content

Introduction to electron tomography
Linux basics
Immersion freezing for cryo-electron tomography
High pressure freezing and CEMOVIS or Cryo-FIB
Cryo-CLEM
SerialEM
Low-dose data collection with SerialEM
Direct electron detectors
Cryo-STEM tomography
Data processing with IMOD and eTomo
Processing of low-dose tilt series with IMOD
Modelling and interpretation of cryo-electron tomography data
Subtomogram averaging with PEET and other packages

Impressions from the 2015, 2017, and 2019 Workshops

(Photos: Harald Kotisch, Guenter Resch, Erin Telley, Nicole Drexler)

Organisers

Thomas Heuser, Vienna Biocenter Core Facilities, Vienna, Austria
Guenter Resch, Nexperion e.U. - Solutions for Electron Microscopy, Vienna, Austria
Florian Schur, Institute of Science and Technology Austria

Instructors

Tommaso Costanzo, Institute of Science and Technology Austria
Florian Faessler, Institute of Science and Technology Austria
Helmut Gnaegi, Diatome, Switzerland
Wim Hagen, EMBL Heidelberg, Germany
David Mastronarde, University of Colorado, Boulder, United States
Christiane Riedel, University of Veterinary Medicine, Vienna, Austria
Sharon Grayer Wolf, Weizmann Institute of Science, Rehovot, Israel
Bettina Zens, Institute of Science and Technology Austria

CO₂ emissions caused by air travel of instructors will be compensated for with a donation to a project funding reforestation and energy efficient cooking systems in Nepal.

Infrastructure

The VBCF EM Facility at the Vienna Biocenter, where the event will be held, is one of the leading electron microscopy facilities in life sciences in Austria and perfectly equipped to hosting an advanced cryo-electron tomography workshop. Dediciated cryo-instrumentation on site includes a ThermoFisher Scientific Glacios electron microscope, Leica EM GP immersion freezer and high pressure freezers, as well as appropriate image processing capacities. Installation of a ThermoFisher Scientific Titan Krios G4 with Selectris energy filter and Falcon4 direct electron detector was completed in early 2022.

One day of the workshop will take place at the Institute of Science and Technology Austria (ISTA), located in Maria Gugging, Klosterneuburg, 20 km northwest of Vienna.
ISTA is an international research institute in natural and mathematical sciences committed to performing world-class research, with several scientific service units (SSUs) providing excellent know-how and cutting-edge instrumentation to support research. ISTA’s cryo-EM facility is equipped with a 300 kV Thermo Scientific Krios TEM (including a Volta phase plate, a post-GIF energy filtered Gatan K3, and a Falcon 3 DED), a 200 kV Thermo Scientific Glacios TEM (including a Volta phase plate and Falcon 3 DED) and a Thermo Scientific Aquilos 2 cryo-FIBSEM, including lift-out capabilities. amples can be frozen by high pressure freezing (BalTec HPM010) and by plunge freezing (Leica EM GP2, TFS Vitrobot Mark IV) methods, cryo-correlative light and electron microscopy experiments can be performed on a Leica cryo-CLEM setup.