The Elec­tron Microscopy Facil­ity of the Vienna Bio­cen­ter Core Facil­i­ties (VBCF, Vienna, Aus­tria), the Schur Lab at IST Aus­tria, and Nex­pe­rion – Solu­tions for Elec­tron Microscopy (Vienna, Aus­tria) are jointly organ­is­ing an inter­na­tional

Advanced Work­shop on Cryo-Electron Tomog­ra­phy

Sat­ur­day, May 7 – Fri­day, May 13, 2022

Vienna, Aus­tria, Europe (on-site)

for stu­dents, post­doc­toral staff, sci­en­tists, and group lead­ers from acad­e­mia and indus­try.


(To be con­firmed)

  • Intro­duc­tion to elec­tron tomog­ra­phy
  • Linux basics
  • Immer­sion freez­ing for cryo-electron tomog­ra­phy
  • High pres­sure freez­ing and CEMO­VIS or Cryo-FIB
  • Cryo-CLEM
  • Seri­alEM
  • Low-dose data col­lec­tion with Seri­alEM
  • Direct elec­tron detec­tors
  • Cryo-STEM tomog­ra­phy
  • Data pro­cess­ing with IMOD and eTomo
  • Pro­cess­ing of low-dose tilt series with IMOD
  • Mod­el­ling and inter­pre­ta­tion of cryo-electron tomog­ra­phy data
  • Subto­mo­gram aver­ag­ing with PEET and other pack­ages

Impres­sions from the 2015, 2017, and 2019 Work­shops

(Pho­tos: Har­ald Kotisch, Guenter Resch, Erin Tel­ley, Nicole Fell­ner)


  • Thomas Heuser, Vienna Bio­cen­ter Core Facil­i­ties, Vienna, Aus­tria
  • Guenter Resch, Nex­pe­rion e.U. — Solu­tions for Elec­tron Microscopy, Vienna, Aus­tria
  • Flo­rian Schur, Insti­tute of Sci­ence and Tech­nol­ogy Aus­tria


CO2 emis­sions caused by air travel of instruc­tors will be com­pen­sated for with a dona­tion to a project fund­ing refor­esta­tion and energy effi­cient cook­ing sys­tems in Nepal.

Spon­sored Guest Lec­tures


The VBCF EM Facil­ity at the Vienna Bio­cen­ter, where the event will be held, is one of the lead­ing elec­tron microscopy facil­i­ties in life sci­ences in Aus­tria and per­fectly equipped to host­ing an advanced cryo-electron tomog­ra­phy work­shop.

Ded­i­cated cryo-instrumentation on site includes a Ther­moFisher Sci­en­tific Glacios elec­tron micro­scope, Leica EM GP immer­sion freezer and high pres­sure freez­ers, as well as appro­pri­ate image pro­cess­ing capac­i­ties. Instal­la­tion of a Ther­moFisher Sci­en­tific Titan Krios G4 with Selec­tris energy fil­ter and Falcon4 direct elec­tron detec­tor is sched­uled to be com­pleted in Jan­u­ary 2022.


The “Advanced Work­shop on Cryo-Electron Tomog­ra­phy” is an event offi­cially sup­ported by the Euro­pean Microscopy Soci­ety (EMS).

European Microscopy Society

The work­shop is gen­er­ously spon­sored by AMETEK/Gatan, Diatome, JEOL Ger­many, Ther­moFisher Sci­en­tific, TVIPS, Alve­ole, Sci­ence Ser­vices, Quan­tifoil and Ams­ter­dam Sci­en­tific Instru­ments.


ThermoFisher ScientificTVIPSAlveole

Science Services Quantifoil


Par­tic­i­pants and Reg­is­tra­tion

A min­i­mum num­ber of 12 up to a max­i­mum of 18 par­tic­i­pants will be allowed to the work­shop, with reg­is­tra­tions being con­sid­ered depend­ing on rel­e­vance, access to infra­struc­ture, and the order of sub­mis­sion. Stu­dents and mem­bers of the Euro­pean Microscopy Soci­ety (EMS) are eli­gi­ble for a reduced reg­is­tra­tion fee:

  • Acad­e­mia reg­is­tra­tion fee: 1400 Euro excl. VAT
  • Indus­try reg­is­tra­tion fee: 2050 Euro excl. VAT
  • Reduced reg­is­tra­tion fee: 1200 Euro excl. VAT

The reg­is­tra­tion fee cov­ers the work­shop fee, work­shop mate­ri­als, cater­ing dur­ing the day includ­ing lunch, and two evening events (city tour and work­shop din­ner).

Please sub­mit your appli­ca­tion until Jan­u­ary 31, 2022 via the appli­ca­tion form.

In order to make the work­shop as relaxed and and worry-free as pos­si­ble and to min­imise poten­tial legal lim­i­ta­tions, we have decided that a valid vac­ci­na­tion against COVID-19 (with a vac­cine approved by the EMA) or a proof of recent recov­ery from the dis­ease is required for every­one par­tic­i­pat­ing (i.e., instruc­tors, par­tic­i­pants, com­pany staff, and other staff).

For gen­eral inquiries, please con­tact This email address is being pro­tected from spam­bots. You need JavaScript enabled to view it., to stay up to date with future events organ­ised by Nex­pe­rion, sub­scribe to the Newslet­ter.


This web­site rep­re­sents the cur­rent state of plan­ning for this event, con­tent and speak­ers are sub­ject to change as plan­ning pro­gresses.