The Elec­tron Microscopy Facil­ity of the Vienna Bio­cen­ter Core Facil­i­ties (VBCF, Vienna, Aus­tria), the Schur Lab at Insti­tute of Sci­ence and Tech­nol­ogy Aus­tria, and Nex­pe­rion – Solu­tions for Elec­tron Microscopy (Vienna, Aus­tria) have jointly organ­ised an inter­na­tional

Advanced Work­shop on Cryo-Electron Tomog­ra­phy

Sat­ur­day, May 7 – Fri­day, May 13, 2022

Vienna, Aus­tria, Europe (on-site)

for stu­dents, post­doc­toral staff, sci­en­tists, and group lead­ers from acad­e­mia and indus­try. This work­shop is sup­ported by the Elec­tron Microscopy Facil­ity at ISTA.

Con­tent

• Intro­duc­tion to elec­tron tomog­ra­phy
• Linux basics
• Immer­sion freez­ing for cryo-electron tomog­ra­phy
• High pres­sure freez­ing and CEMO­VIS or Cryo-FIB
• Cryo-CLEM
• Seri­alEM
• Low-dose data col­lec­tion with Seri­alEM
• Direct elec­tron detec­tors
• Cryo-STEM tomog­ra­phy
• Data pro­cess­ing with IMOD and eTomo
• Pro­cess­ing of low-dose tilt series with IMOD
• Mod­el­ling and inter­pre­ta­tion of cryo-electron tomog­ra­phy data
• Subto­mo­gram aver­ag­ing with PEET and other pack­ages

Impres­sions from the 2015, 2017, and 2019 Work­shops

(Pho­tos: Har­ald Kotisch, Guenter Resch, Erin Tel­ley, Nicole Fell­ner)

Organ­is­ers

• Thomas Heuser, Vienna Bio­cen­ter Core Facil­i­ties, Vienna, Aus­tria
• Guenter Resch, Nex­pe­rion e.U. — Solu­tions for Elec­tron Microscopy, Vienna, Aus­tria
• Flo­rian Schur, Insti­tute of Sci­ence and Tech­nol­ogy Aus­tria

Instruc­tors

• Tom­maso Costanzo, Insti­tute of Sci­ence and Tech­nol­ogy Aus­tria
• Flo­rian Faessler, Insti­tute of Sci­ence and Tech­nol­ogy Aus­tria
• Hel­mut Gnaegi, Diatome, Switzer­land
• Wim Hagen, EMBL Hei­del­berg, Ger­many
• David Mas­tronarde, Uni­ver­sity of Col­orado, Boul­der, United States
• Chris­tiane Riedel, Uni­ver­sity of Vet­eri­nary Med­i­cine, Vienna, Aus­tria
• Sharon Grayer Wolf, Weiz­mann Insti­tute of Sci­ence, Rehovot, Israel
• Bet­tina Zens, Insti­tute of Sci­ence and Tech­nol­ogy Aus­tria

CO₂ emis­sions caused by air travel of instruc­tors will be com­pen­sated for with a dona­tion to a project fund­ing refor­esta­tion and energy effi­cient cook­ing sys­tems in Nepal.

Spon­sored Guest Lec­tures

• Shee­tal Bhard­waj, Ams­ter­dam Sci­en­tific Instru­ments
• Alex­ane Caig­nard, Alve­ole
• Eric Hum­mel, Leica Microsys­tems
• Andreas Kas­ten­müller, AME­TEK GmbH, Busi­ness Unit Gatan
• Georg Raggl, JEOL (Ger­many) GmbH
• Marit Smeets, Delmic
• Cveta Tomova, Thermo Sci­en­tific
• Tia Tru­glas, TVIPS
• Eric Hum­mel, Leica Microsys­tems

Infra­struc­ture

The VBCF EM Facil­ity at the Vienna Bio­cen­ter, where the event will be held, is one of the lead­ing elec­tron microscopy facil­i­ties in life sci­ences in Aus­tria and per­fectly equipped to host­ing an advanced cryo-electron tomog­ra­phy work­shop. Ded­i­ciated cryo-instrumentation on site includes a Ther­moFisher Sci­en­tific Glacios elec­tron micro­scope, Leica EM GP immer­sion freezer and high pres­sure freez­ers, as well as appro­pri­ate image pro­cess­ing capac­i­ties. Instal­la­tion of a Ther­moFisher Sci­en­tific Titan Krios G4 with Selec­tris energy fil­ter and Falcon4 direct elec­tron detec­tor was com­pleted in early 2022.

One day of the work­shop will take place at the Insti­tute of Sci­ence and Tech­nol­ogy Aus­tria (ISTA), located in Maria Gug­ging, Klosterneuburg, 20 km north­west of Vienna.
ISTA is an inter­na­tional research insti­tute in nat­ural and math­e­mat­i­cal sci­ences com­mit­ted to per­form­ing world-class research, with sev­eral sci­en­tific ser­vice units (SSUs) pro­vid­ing excel­lent know-how and cutting-edge instru­men­ta­tion to sup­port research. ISTA’s cryo-EM facil­ity is equipped with a 300 kV Thermo Sci­en­tific Krios TEM (includ­ing a Volta phase plate, a post-GIF energy fil­tered Gatan K3, and a Fal­con 3 DED), a 200 kV Thermo Sci­en­tific Glacios TEM (includ­ing a Volta phase plate and Fal­con 3 DED) and a Thermo Sci­en­tific Aqui­los 2 cryo-FIBSEM, includ­ing lift-out capa­bil­i­ties. amples can be frozen by high pres­sure freez­ing (Bal­Tec HPM010) and by plunge freez­ing (Leica EM GP2, TFS Vit­ro­bot Mark IV) meth­ods, cryo-correlative light and elec­tron microscopy exper­i­ments can be per­formed on a Leica cryo-CLEM setup.

Spon­sors

The “Advanced Work­shop on Cryo-Electron Tomog­ra­phy” is an event offi­cially sup­ported by the Euro­pean Microscopy Soci­ety (EMS).

The work­shop was gen­er­ously spon­sored by AMETEK/Gatan, Diatome, Leica Microsys­tems, Delmic, JEOL Ger­many, Ther­moFisher Sci­en­tific, TVIPS, Alve­ole, Sci­ence Ser­vices, Quan­tifoil and Ams­ter­dam Sci­en­tific Instru­ments.

Par­tic­i­pants and Reg­is­tra­tion

The appli­ca­tion for the work­shop is closed; all appli­cants were informed in early Feb­ru­ary 2022 about the result of the appli­ca­tion pro­ce­dure.

For gen­eral inquiries, please con­tact This email address is being pro­tected from spam­bots. You need JavaScript enabled to view it., to stay up to date with future events organ­ised by Nex­pe­rion, sub­scribe to the Newslet­ter.