Seri­alEM

Seri­alEM is a soft­ware devel­oped by Dr. David Mas­tronarde (at the Uni­ver­sity of Col­orado and for­merly part of The Boul­der Lab for 3D Elec­tron Microscopy of Cells) enabling auto­mated acqui­si­tion of a vari­ety of data from trans­mis­sion elec­tron micro­scopes: tilt series for (cryo-)electron tomog­ra­phy, large image areas for 3D recon­struc­tion from ser­ial sec­tions, and — via its pow­er­ful script­ing lan­guage — images for recon­struc­tion of macro­mol­e­cules by single-particle meth­ods.

Seri­alEM is com­pat­i­ble with devices from major man­u­fac­tur­ers such as Thermo Scientific/FEI Tec­nai, Titan, and Talos micro­scopes with the TEM script­ing adapter, JEOL TEMs with the TEM­CON or TEM­CEN­TER inter­face, the Hitachi HT7700/7800, and cameras/detectors from all major brands.

In col­lab­o­ra­tion with SerialEM’s author, Nex­pe­rion pro­vides sup­port and resources for this tool, and man­ages the finan­cial resources for future devel­op­ment of the soft­ware. All prob­lem reports and requests should be ini­tially sent to This email address is being pro­tected from spam­bots. You need JavaScript enabled to view it., which is mon­i­tored by Nex­pe­rion.

The instal­la­tion and cal­i­bra­tion of Seri­alEM is avail­able as a ser­vice by Nex­pe­rion for all sup­ported plat­forms, both on-site as well as remotely.

Read more

Nex­pe­rion pro­vides on-site and remote train­ing on Seri­alEM, typ­i­cally in con­junc­tion with an instal­la­tion or as a part of a big­ger event.

Read more

A repos­i­tory for shar­ing Seri­alEM scripts (macros) allow­ing automa­tion of TEM data acqui­si­tion is being pro­vided by Nex­pe­rion.

Read more

Reg­is­ter here for a Nex­pe­rion Ser­vice to receive auto­matic email noti­fi­ca­tions once a Seri­alEM ver­sion is pub­lished.

Read more

To enable user sup­port and future devel­op­ment of Seri­alEM, Nex­pe­rion is man­ag­ing a fund of sup­port pay­ments on behalf of the devel­oper.

Read more