The Elec­tron Microscopy Facil­ity of the Vienna Bio­cen­ter Core Facil­i­ties (VBCF, Vienna, Aus­tria) and Nex­pe­rion – Solu­tions for Elec­tron Microscopy (Vienna, Aus­tria) have jointly organ­ised an inter­na­tional

Advanced Work­shop on Cryo-Electron Tomog­ra­phy

Vienna, Aus­tria, Europe

May 67, 2017: Optional Pre-Course
May 812, 2017: Work­shop

for stu­dents, post­doc­toral staff, sci­en­tists, and group lead­ers from acad­e­mia and indus­try.

While the work­shop was tar­get­ing an advanced audi­ence (pre-existing knowl­edge in elec­tron tomog­ra­phy, data pro­cess­ing, and/or cryo-TEM), a week­end pre-course was be offered for less expe­ri­enced par­tic­i­pants to catch-up.

Impres­sions from the Work­shop

(Group photo: Har­ald Kotisch; din­ner pho­tos: Sharon Grayer Wolf)

Con­tent Pre-Course (Sat/Sun, May 67, 2017)

(Optional for expe­ri­enced par­tic­i­pants)

  • Intro­duc­tion to elec­tron tomog­ra­phy and Seri­alEM
  • Linux
  • Data pro­cess­ing with IMOD and eTomo

Con­tent Work­shop (Mon-Fri, May 812, 2017)

  • Immer­sion freez­ing for cryo-electron tomog­ra­phy
  • High pres­sure freez­ing and CEMO­VIS
  • Cryo-CLEM
  • Low-dose data col­lec­tion with Seri­alEM
  • Direct elec­tron detec­tors
  • Cryo-STEM tomog­ra­phy
  • Pro­cess­ing of low-dose tilt series with IMOD
  • Mod­el­ling and inter­pre­ta­tion of cryo-electron tomog­ra­phy data
  • Subto­mo­gram aver­ag­ing with PEET

Instruc­tors and Organ­is­ers

  • Dr. Thomas Heuser, Vienna Bio­cen­ter Core Facil­i­ties, Vienna, Aus­tria
  • Dr. Guenter Resch, Nex­pe­rion e.U. — Solu­tions for Elec­tron Microscopy, Vienna, Aus­tria
  • Dr. Mikhail Eltsov, Buch­mann Insti­tute for Mol­e­c­u­lar Life Sci­ences, Goethe Uni­ver­sity Frank­furt, Ger­many
  • Dr. Johanna Höög, Uni­ver­sity of Gothen­burg, Swe­den
  • Dr. David Mas­tronarde, Uni­ver­sity of Col­orado, Boul­der, United States
  • Dr. Rein­hard Rachel, Uni­ver­sity of Regens­burg, Ger­many
  • Dr. Chris­tiane Riedel, Vet­er­inärmedi­zinis­che Uni­ver­sität Wien, Aus­tria
  • Dr. Sharon Grayer Wolf, Weiz­mann Insti­tute of Sci­ence, Rehovot, Israel

FEI Tecnai Helium (Polara)

Spon­sored Guest Lec­tures

Infra­struc­ture

The VBCF EM Facil­ity at the Vienna Bio­cen­ter, where the event was held, is one of the lead­ing elec­tron microscopy facil­i­ties in life sci­ences in Aus­tria and per­fectly equipped to host­ing an advanced cryo-electron tomog­ra­phy work­shop.

Ded­i­cated cryo-instrumentation includ­ing an FEI Tec­nai Polara Helium elec­tron micro­scope with a Gatan K2 Sum­mit Direct Elec­tron detec­tor and a post-column energy fil­ter, a Leica EM GP immer­sion freezer, a Leica EM Cryo-CLEM sys­tem, as well as appro­pri­ate image pro­cess­ing capac­i­ties were avail­able on site.

Spon­sors

The “Advanced Work­shop on Cryo-Electron Tomog­ra­phy” is an event offi­cially sup­ported by the Euro­pean Microscopy Soci­ety (EMS).

European Microscopy Society

 The work­shop is gen­er­ously spon­sored by Leica Microsys­tems, Gatan, Diatome, FEI, JEOL Ger­many, Sci­ence Ser­vices, and Quan­tifoil.

Leica MicrosystemsGatanDiatomeFEI

JEOLScience Services Quantifoil

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