Liq­uid nitro­gen and liq­uid ethane/propane are com­monly used cryo­gens in cryo-electron microscopy and many EM spec­i­men prepa­ra­tion tech­niques, such as high pres­sure freez­ing and freeze sub­sti­tu­tion. When­ever cry­otech­niques are taught, an overview of the safe han­dling of cryogens is necessary.

Nex­pe­rion is happy to share its safety teach­ing mate­r­ial with the com­mu­nity. Slides can be either viewed indi­vid­u­ally or a pre­sen­ta­tion in PDF for­mat can be down­loaded (1.8 MB) and used accord­ing to Cre­ative Com­mons license BY-ND.

It is also worth point­ing out that our mate­r­ial is intended to com­ple­ment local and insti­tu­tional safety reg­u­la­tions. No lia­bil­ity can be taken for the infor­ma­tion pro­vided, feed­back from col­leagues is wel­come.