Liquid nitrogen and liquid ethane/propane are commonly used cryogens in cryo-electron microscopy and many EM specimen preparation techniques, such as high pressure freezing and freeze substitution. Whenever cryotechniques are taught, an overview of the safe handling of cryogens is necessary.
Nexperion is happy to share its safety teaching material with the community. Slides can be either viewed individually or a presentation in PDF format can be downloaded (1.8 MB) and used according to Creative Commons license BY-ND.
It is also worth pointing out that our material is intended to complement local and institutional safety regulations. No liability can be taken for the information provided, feedback from colleagues is welcome.