The Elec­tron Microscopy Facil­ity of the Vienna Bio­cen­ter Core Facil­i­ties (VBCF, Vienna, Aus­tria), the Schur Lab at IST Aus­tria, and Nex­pe­rion – Solu­tions for Elec­tron Microscopy (Vienna, Aus­tria) are jointly plan­ning an inter­na­tional

Advanced Work­shop on Cryo-Electron Tomog­ra­phy

Vienna, Aus­tria, Europe

May 1521, 2021

for stu­dents, post­doc­toral staff, sci­en­tists, and group lead­ers from acad­e­mia and indus­try.

Con­tent

(To be con­firmed)

  • Intro­duc­tion to elec­tron tomog­ra­phy
  • Immer­sion freez­ing for cryo-electron tomog­ra­phy
  • High pres­sure freez­ing and CEMO­VIS or Cryo-FIB
  • Cryo-CLEM
  • Seri­alEM
  • Low-dose data col­lec­tion with Seri­alEM
  • Direct elec­tron detec­tors
  • Cryo-STEM tomog­ra­phy
  • Linux basics
  • Data pro­cess­ing with IMOD and eTomo
  • Pro­cess­ing of low-dose tilt series with IMOD
  • Mod­el­ling and inter­pre­ta­tion of cryo-electron tomog­ra­phy data
  • Subto­mo­gram aver­ag­ing with PEET

Impres­sions from the 2015, 2017, and 2019 Work­shops

(Pho­tos: Har­ald Kotisch, Guenter Resch, Erin Tel­ley, Nicole Fell­ner)

Organ­is­ers

  • Thomas Heuser, Vienna Bio­cen­ter Core Facil­i­ties, Vienna, Aus­tria
  • Guenter Resch, Nex­pe­rion e.U. — Solu­tions for Elec­tron Microscopy, Vienna, Aus­tria
  • Flo­rian Schur, Insti­tute of Sci­ence and Tech­nol­ogy Aus­tria

Instruc­tors and Spon­sored Guest Lec­tures

(To be con­firmed)

  • Flo­rian Faessler, Insti­tute of Sci­ence and Tech­nol­ogy Aus­tria
  • Wim Hagen, EMBL Hei­del­berg, Ger­many
  • David Mas­tronarde, Uni­ver­sity of Col­orado, Boul­der, United States
  • Chris­tiane Riedel, Vet­er­inärmedi­zinis­che Uni­ver­sität Wien, Aus­tria
  • Sharon Grayer Wolf, Weiz­mann Insti­tute of Sci­ence, Rehovot, Israel

Infra­struc­ture

The VBCF EM Facil­ity at the Vienna Bio­cen­ter, where the event will be held, is one of the lead­ing elec­tron microscopy facil­i­ties in life sci­ences in Aus­tria and per­fectly equipped to host­ing an advanced cryo-electron tomog­ra­phy work­shop.

Ded­i­cated cryo-instrumentation includ­ing an Ther­moFisher Sci­en­tific Glacios elec­tron micro­scope with a Fal­con 3EC Direct Elec­tron detec­tor, a Leica EM GP immer­sion freezer, a Leica EM Cryo-CLEM sys­tem, as well as appro­pri­ate image pro­cess­ing capac­i­ties are avail­able on site.

Par­tic­i­pants and Reg­is­tra­tion

The reg­is­tra­tion for the work­shop will open end of 2020.

Due to the ongo­ing pan­demic poten­tially affect­ing par­tic­i­pants, instruc­tors, and venue alike, the final deci­sion about the event will be made later — all par­tic­i­pants are asked to take this into account when plan­ning their travel. An online event to replace the work­shop is not planned, as we con­sider the direct inter­ac­tion between par­tic­i­pants and teach­ers as well as the oppor­tu­nity to prac­tice on-site essen­tial for this for­mat.

For gen­eral inquiries, please con­tact This email address is being pro­tected from spam­bots. You need JavaScript enabled to view it., to stay up to date with future events organ­ised by Nex­pe­rion, sub­scribe to the Newslet­ter.

Spon­sors

The “Advanced Work­shop on Cryo-Electron Tomog­ra­phy” is an event offi­cially sup­ported by the Euro­pean Microscopy Soci­ety (EMS).

European Microscopy Society

Dis­claimer

This web­site rep­re­sents the cur­rent state of plan­ning for this event, con­tent and speak­ers are sub­ject to change as plan­ning pro­gresses.