The Elec­tron Microscopy Facil­ity of the Vienna Bio­cen­ter Core Facil­i­ties (VBCF, Vienna, Aus­tria) and Nex­pe­rion – Solu­tions for Elec­tron Microscopy (Vienna, Aus­tria) are jointly organ­is­ing an inter­na­tional

Advanced Work­shop on Cryo-Electron Tomog­ra­phy

Vienna, Aus­tria, Europe

May 1112, 2019: Optional Pre-Course
May 1217, 2019: Work­shop

for stu­dents, post­doc­toral staff, sci­en­tists, and group lead­ers from acad­e­mia and indus­try.

While the work­shop is tar­get­ing an advanced audi­ence (pre-existing knowl­edge in elec­tron tomog­ra­phy, data pro­cess­ing, and/or cryo-TEM), a week­end pre-course will be offered for less expe­ri­enced par­tic­i­pants to catch-up.

Impres­sions from the 2015 and 2017 Work­shops

(Pho­tos: Har­ald Kotisch, Guenter Resch, Erin Tel­ley)

Con­tent Pre-Course (Sat/Sun, May 1112, 2019)

(Optional for expe­ri­enced par­tic­i­pants)

  • Intro­duc­tion to elec­tron tomog­ra­phy and Seri­alEM
  • Linux
  • Data pro­cess­ing with IMOD and eTomo

Con­tent Work­shop (Mon-Fri, May 1317, 2019)

  • Spec­i­men prepa­ra­tion tech­niques for cryo-electron tomog­ra­phy
  • Cryo-CLEM
  • Low-dose data col­lec­tion with Seri­alEM
  • Direct elec­tron detec­tors
  • Cryo-STEM tomog­ra­phy
  • Pro­cess­ing of low-dose tilt series with IMOD
  • Mod­el­ling and inter­pre­ta­tion of cryo-electron tomog­ra­phy data
  • Subto­mo­gram aver­ag­ing with PEET

Instruc­tors, Spon­sored Guest Lec­tures, and Organ­is­ers

To be con­firmed


The VBCF EM Facil­ity at the Vienna Bio­cen­ter, where the event will be held, is one of the lead­ing elec­tron microscopy facil­i­ties in life sci­ences in Aus­tria and per­fectly equipped to host­ing an advanced cryo-electron tomog­ra­phy work­shop.

Ded­i­cated cryo-instrumentation includ­ing an Ther­moFisher Sci­en­tific Glacios elec­tron micro­scope with a Fal­con 3EC Direct Elec­tron detec­tor, a Leica EM GP immer­sion freezer, a Leica EM Cryo-CLEM sys­tem, as well as appro­pri­ate image pro­cess­ing capac­i­ties are avail­able on site.

Par­tic­i­pants and Reg­is­tra­tion

The reg­is­tra­tion for the work­shop will open end of 2018.

For gen­eral inquiries, please con­tact This email address is being pro­tected from spam­bots. You need JavaScript enabled to view it., to stay up to date with future events organ­ised by Nex­pe­rion, sub­scribe to the Newslet­ter.


The “Advanced Work­shop on Cryo-Electron Tomog­ra­phy” is an event offi­cially sup­ported by the Euro­pean Microscopy Soci­ety (EMS).

European Microscopy Society


This web­site rep­re­sents the cur­rent state of plan­ning for this event, con­tent and speak­ers are sub­ject to change as plan­ning pro­gresses.