The Elec­tron Microscopy Facil­ity of the Vienna Bio­cen­ter Core Facil­i­ties (VBCF, Vienna, Aus­tria) and Nex­pe­rion – Solu­tions for Elec­tron Microscopy (Vienna, Aus­tria) were jointly organ­is­ing an inter­na­tional

Advanced Work­shop on Cryo-Electron Tomog­ra­phy

Vienna, Aus­tria, Europe

May 1112, 2019: Optional Pre-Course
May 1317, 2019: Work­shop

for stu­dents, post­doc­toral staff, sci­en­tists, and group lead­ers from acad­e­mia and indus­try.

While the work­shop was tar­get­ing an advanced audi­ence (pre-existing knowl­edge in elec­tron tomog­ra­phy, data pro­cess­ing, and/or cryo-TEM), a week­end pre-course was be offered for less expe­ri­enced par­tic­i­pants to catch-up.

Impres­sions from the 2015 and 2017 Work­shops

(Pho­tos: Har­ald Kotisch, Guenter Resch, Erin Tel­ley)

Con­tent Pre-Course (Sat/Sun, May 1112, 2019)

(Rec­om­mended for less expe­ri­enced par­tic­i­pants)

  • Intro­duc­tion to elec­tron tomog­ra­phy and Seri­alEM
  • Linux
  • Data pro­cess­ing with IMOD and eTomo

Con­tent Work­shop (Mon-Fri, May 1317, 2019)

  • Spec­i­men prepa­ra­tion tech­niques for cryo-electron tomog­ra­phy
  • Cryo-CLEM
  • Low-dose data col­lec­tion with Seri­alEM
  • Direct elec­tron detec­tors
  • Cryo-STEM tomog­ra­phy
  • Pro­cess­ing of low-dose tilt series with IMOD
  • Mod­el­ling and inter­pre­ta­tion of cryo-electron tomog­ra­phy data
  • Subto­mo­gram aver­ag­ing with PEET

Instruc­tors and Organ­is­ers

  • Dr. Ben Engel, Max Planck Insti­tute of Bio­chem­istry , Mar­tin­sried, Ger­many
  • Dr. Thomas Heuser, Vienna Bio­cen­ter Core Facil­i­ties, Vienna, Aus­tria
  • Dr. David Mas­tronarde, Uni­ver­sity of Col­orado, Boul­der, United States
  • Dr. Guenter Resch, Nex­pe­rion e.U. — Solu­tions for Elec­tron Microscopy, Vienna, Aus­tria
  • Dr. Chris­tiane Riedel, Vet­er­inärmedi­zinis­che Uni­ver­sität Wien, Aus­tria
  • Dr. Flo­rian Schur, Insti­tute of Sci­ence and Tech­nol­ogy, Klosterneuburg, Aus­tria
  • Dr. Sharon Grayer Wolf, Weiz­mann Insti­tute of Sci­ence, Rehovot, Israel

Spon­sored Guest Lec­tures

  • Mr. Hel­mut Gnaegi, Diatome Ltd., Switzer­land
  • Dr. Emanuel Katz­mann, JEOL (Ger­many) GmbH
  • Dr. Andreas Kas­ten­müller, Gatan GmbH
  • Dr. Frédéric Ler­oux, Leica Microsys­tems
  • Dr Max Maletta, Thermo Sci­en­tific
  • Ms. Kristýna Rosı́ková, TES­CAN Brno, s.r.o.
  • Dr. Andreas Wis­net, TVIPS GmbH
  • Mr. Jan de Weert, Leica Microsys­tems

Infra­struc­ture

The VBCF EM Facil­ity at the Vienna Bio­cen­ter, where the event was held, is one of the lead­ing elec­tron microscopy facil­i­ties in life sci­ences in Aus­tria and per­fectly equipped to host­ing an advanced cryo-electron tomog­ra­phy work­shop.

Ded­i­cated cryo-instrumentation includ­ing an Ther­moFisher Sci­en­tific Glacios elec­tron micro­scope with a Fal­con 3EC Direct Elec­tron detec­tor, a Leica EM GP immer­sion freezer, a Leica EM Cryo-CLEM sys­tem, as well as appro­pri­ate image pro­cess­ing capac­i­ties were avail­able on site.

Spon­sors

The “Advanced Work­shop on Cryo-Electron Tomog­ra­phy” was an event offi­cially sup­ported by the Euro­pean Microscopy Soci­ety (EMS).

European Microscopy Society

The work­shop was gen­er­ously spon­sored by Tes­can, Gatan, Diatome, Ther­moFisher Sci­en­tific, JEOL Ger­many, Leica Microsys­tems, TVIPS, and Sci­ence Ser­vices.

TESCANGatanDiatome

ThermoFisher ScientificJEOLLeica Microsystems

TVIPSScience Services