The Elec­tron Microscopy Facil­ity of the Vienna Bio­cen­ter Core Facil­i­ties (VBCF, Vienna, Aus­tria) and Nex­pe­rion – Solu­tions for Elec­tron Microscopy (Vienna, Aus­tria) are jointly organ­is­ing an inter­na­tional

Advanced Work­shop on Cryo-Electron Tomog­ra­phy

Vienna, Aus­tria, Europe

May 1112, 2019: Optional Pre-Course
May 1317, 2019: Work­shop

for stu­dents, post­doc­toral staff, sci­en­tists, and group lead­ers from acad­e­mia and indus­try.

While the work­shop is tar­get­ing an advanced audi­ence (pre-existing knowl­edge in elec­tron tomog­ra­phy, data pro­cess­ing, and/or cryo-TEM), a week­end pre-course will be offered for less expe­ri­enced par­tic­i­pants to catch-up.

Impres­sions from the 2015 and 2017 Work­shops

(Pho­tos: Har­ald Kotisch, Guenter Resch, Erin Tel­ley)

Con­tent Pre-Course (Sat/Sun, May 1112, 2019)

(Rec­om­mended for less expe­ri­enced par­tic­i­pants)

  • Intro­duc­tion to elec­tron tomog­ra­phy and Seri­alEM
  • Linux
  • Data pro­cess­ing with IMOD and eTomo

Con­tent Work­shop (Mon-Fri, May 1317, 2019)

  • Spec­i­men prepa­ra­tion tech­niques for cryo-electron tomog­ra­phy
  • Cryo-CLEM
  • Low-dose data col­lec­tion with Seri­alEM
  • Direct elec­tron detec­tors
  • Cryo-STEM tomog­ra­phy
  • Pro­cess­ing of low-dose tilt series with IMOD
  • Mod­el­ling and inter­pre­ta­tion of cryo-electron tomog­ra­phy data
  • Subto­mo­gram aver­ag­ing with PEET

Instruc­tors and Organ­is­ers

  • Dr. Thomas Heuser, Vienna Bio­cen­ter Core Facil­i­ties, Vienna, Aus­tria
  • Dr. David Mas­tronarde, Uni­ver­sity of Col­orado, Boul­der, United States
  • Dr. Guenter Resch, Nex­pe­rion e.U. — Solu­tions for Elec­tron Microscopy, Vienna, Aus­tria
  • Dr. Chris­tiane Riedel, Vet­er­inärmedi­zinis­che Uni­ver­sität Wien, Aus­tria
  • Dr. Flo­rian Schur, Insti­tute of Sci­ence and Tech­nol­ogy, Klosterneuburg, Aus­tria
  • Dr. Sharon Grayer Wolf, Weiz­mann Insti­tute of Sci­ence, Rehovot, Israel
  • Mr. Hel­mut Gnaegi, Diatome Ltd., Switzer­land

Infra­struc­ture

The VBCF EM Facil­ity at the Vienna Bio­cen­ter, where the event will be held, is one of the lead­ing elec­tron microscopy facil­i­ties in life sci­ences in Aus­tria and per­fectly equipped to host­ing an advanced cryo-electron tomog­ra­phy work­shop.

Ded­i­cated cryo-instrumentation includ­ing an Ther­moFisher Sci­en­tific Glacios elec­tron micro­scope with a Fal­con 3EC Direct Elec­tron detec­tor, a Leica EM GP immer­sion freezer, a Leica EM Cryo-CLEM sys­tem, as well as appro­pri­ate image pro­cess­ing capac­i­ties are avail­able on site.

Par­tic­i­pants and Reg­is­tra­tion

A min­i­mum num­ber of 12 up to a max­i­mum of 18 par­tic­i­pants will be allowed to the work­shop, with reg­is­tra­tions being con­sid­ered depend­ing on qual­i­fi­ca­tion and the order of sub­mis­sion. Stu­dents and mem­bers of the Euro­pean Microscopy Soci­ety (EMS) are eli­gi­ble for a reduced reg­is­tra­tion fee:

  • Acad­e­mia reg­is­tra­tion fee: 1000 Euro excl. VAT (1350 Euro excl. VAT with pre-course)
  • Indus­try reg­is­tra­tion fee: 1500 Euro excl. VAT (1950 Euro excl. VAT with pre-course)
  • Reduced reg­is­tra­tion fee: 850 Euro excl. VAT (1150 Euro excl. VAT with pre-course)

The reg­is­tra­tion fee cov­ers the work­shop fee, work­shop mate­ri­als, cater­ing dur­ing the day includ­ing lunch, and two evening events (city tour and work­shop din­ner).

Please send your appli­ca­tion until Jan­u­ary 31, 2019 includ­ing

  • Name
  • Affil­i­a­tion and posi­tion
  • Address for invoic­ing
  • Euro­pean VAT reg­is­tra­tion num­ber of the insti­tu­tion pay­ing the fee, if applic­a­ble
  • Proof of stu­dent sta­tus or EMS mem­ber­ship (if applic­a­ble)
  • Indi­ca­tion of inter­est in optional pre-course
  • Infor­ma­tion about sci­en­tific and tech­ni­cal back­ground, espe­cially about your research or ser­vice project and prior expe­ri­ence with cryo-EM, elec­tron tomog­ra­phy, Seri­alEM, or IMOD
  • Infor­ma­tion about TEM infra­struc­ture acces­si­ble to you

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Spon­sors

The “Advanced Work­shop on Cryo-Electron Tomog­ra­phy” is an event offi­cially sup­ported by the Euro­pean Microscopy Soci­ety (EMS).

European Microscopy Society

The work­shop is gen­er­ously spon­sored by Gatan, Diatome, Ther­moFisher Sci­en­tific, JEOL Ger­many, and Sci­ence Ser­vices.

GatanDiatomeThermoFisher Scientific

JEOLScience Services

Dis­claimer

This web­site rep­re­sents the cur­rent state of plan­ning for this event, con­tent and speak­ers are sub­ject to change as plan­ning pro­gresses.